Progress-curve analysis in enzyme kinetics. Numerical solution of integrated rate equations.
نویسنده
چکیده
Progress curves of enzyme-catalysed reactions are described by equations of a type that precludes direct calculation of the extent of reaction at any time. Previously, such equations have been solved by the Newton-Raphson method, but this procedure may fail when based upon the usual formulae. An alternative formulation is proposed that is both quicker and more robust.
منابع مشابه
Analysis of progress curves for enzyme-catalyzed reactions: application to unstable enzymes, coupled reactions and transient-state kinetics.
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عنوان ژورنال:
- The Biochemical journal
دوره 235 2 شماره
صفحات -
تاریخ انتشار 1986